光遗传学载体

25,6, 2018

光遗传激活病毒列表

遗传激活工具

病毒特点

AAV-CaMKIIa-hChR2(H134R)-mCherry

CaMKIIa启动子(部分脑区为兴奋性神经元特异启动子);使用最普遍的ChR2,激发波长470 nm

AAV-CaMKIIa-hChR2(H134R)-EYFP

AAV-hSyn-hChR2(H134R)-EYFP

hSyn神经元特异启动子;

AAV-hSyn-hChR2(H134R)-mCherry

AAV-CAG-hChR2(H134R)-tdTomato 

CAG非特异强启动子

AAV-CAG-ChR2-GFP

AAV-CMV-bGI-hChR2(H134R)-mCherry

CMV非特异强启动子

AAV-EF1a-double floxed-hChR2(H134R)-mCherry

Cre依赖;Ef1α非神经元特异强启动子;使用最普遍的ChR2,激发波长470 nm;

AAV-EF1a-double floxed-hChR2(H134R)-EYFP

AAV-EF1a-fDIO-hChR2(H134R)-EYFP

flp依赖

AAV-nEF-Con/Fon-hChR2(H134R)-EYFP

Cre或者Flp开启表达;nEF启动子

AAV-hSyn-Con/Foff-hChR2(H134R)-EYFP 

Cre开启,Flp关闭表达

AAV-hSyn-Con/Fon-hChR2(H134R)-EYFP

Cre或者Flp开启表达

AAV-hSyn Coff/Fon hChR2(H134R)-EYFP

Cre关闭,Flp开启表达

AAV-Ef1a-DIO C1V1 (t/t)-TS-EYFP 

C1V1 (t/t)由ChR1和VChR1(ChR的突变体)组成的杂合体,激发光波长560 nm

AAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry

AAV-CaMKIIa-C1V1(t/t)-TS-EYFP

AAV-CaMKIIa-C1V1 (t/t)-TS-mCherry

AAV-CaMKIIa-hChR2(E123A)-EYFP

hChR2(E123A)即ChETA(A)是点突变的ChR2,适合高频激活,激活波长470 nm

AAV-CaMKIIa-hChR2(E123A)-mCherry 

AAV-Ef1a-DIO ChETA-EYFP

Cre依赖ChETA

AAV-CaMKIIa-hChR2(E123T/T159C)-EYFP

hChR2(E123T/T159C)即ChETA(TC)是突变的ChR2,适合高频激活,激活波长470 nm

AAV-CaMKIIa-hChR2(E123T/T159C)-mCherry

AAV-hSyn-oChIEF-tdTomato

CHIEF是ChR1和ChR2的杂合体,可用于高频刺激,激活波长470 nm

AAV-Flex-rev-oChIEF-tdTomato

Cre依赖CHIEF

AAV-hSyn-FAS-DO-ReaChR-mCitrine

ReaChR由ChEF/ChIEF,VChR1,VChR2外加L171I突变组成,激发波长590-630 nm

AAV-Syn-ChrimsonR-GFP

天然存在的CnChR1(Chrimson)的K176R点突变,激发波长590 nm

AAV-hSyn-ChrimsonR-tdTomato

AAV-CaMKIIa-ChrimsonR-tdTomato

AAV-CaMKIIa-ChrimsonR-GFP

AAV-Syn-FLEX-rc[ChrimsonR-tdTomato]

Cre依赖的ChrimsonR

AAV-CaMKII-Chronos-GFP

天然存在的ShChR(Chronos),激发波长530 nm

AAV-Syn-Chronos-GFP


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